* CRITERIA FOR ALL ARMS OF STUDY:
Women of reproductive age will also undergo a pregnancy test (urine hCG) prior to starting the testing procedure. If the woman is determined to be pregnant, she will not be able to participate in this study.
CRITICAL CARE COMPONENT:
INCLUSION CRITERIA:
Diagnosis of sepsis by the primary clinical provider in the ICU.
Diagnosis of the above is based on diagnostic criteria for sepsis as defined by the 2001 SCCM/ESICM/ACCP/ATS/SIS International Sepsis Definitions Conference as listed below:
Infection, documented or suspected, and some of the following:
General variables
* Fever (core temperature greater than 38.3 C)
* Hypothermia (core temperature greater than 36C)
* Heart Rate greater than 90 min or greater than 2 SD above the normal value for age
* Tachypnea
* Altered mental status
* Significant edema or positive fluid balance (greater than 20ml/kg over 24 hours)
* Hyperglycemia (plasma glucose greater than 120 mg/dl) in the absence of diabetes
Inflammatory variables
* Leukocytosis (WBC count greater than 12,000 uL)
* Leukopenia (WBC count less than 4000 uL)
* Normal WBC count with greater than 10 percent immature forms
* Plasma C-reactive protein greater than 2 SD above the normal value
* Plasma procalcitonin greater than 2 SD above the normal value
Hemodynamic variables
* Arterial hypotension (SBP less than 90mm Hg, MAP less than 70, or an SBP decrease greater than 40 mm Hg in adults or less than 2 SD below normal for age)
* SvO2 greater than 70 percent
* Cardiac Index greater than 3.5L min(-1) M(-23)
EXCLUSION CRITERIA:
Pregnancy
Age less than 15 years at the Clinical Center; age less than 18 years at Georgetown or Suburban Hospitals
On glucocorticoids or megace within two weeks of admission unless using more than one of the following medications, patients taking inhaled corticosteriods (less than 1.5 mg/day budesonide, beclomethasone dipropionate and triamcinolone acetonide and less thab 0.75 mg/day for fluticasone propionate) or intranasal corticosteriods will not be excluded.
Patients who have received chronic steroid suppressive medications, i.e. etomidate, ketoconazole (Patients receiving etomidate for intubation purposes as a one time dose will not be excluded)
End stage renal or liver disease (creatinine clearance estimated as less than 20 cc/min by the cockcroft-gault equation: (140 - age) times lean body weight (kg)/ pCr (mg/dl) times 72, in patients with stable renal function; patients requiring dialysis; acute or fulminant hepatitis, alcoholic hepatitis, chronic severe hepatitis, severe obstructive hepatitis, severe coagulopathy, extrahepatic manifestations of ESLD, i.e. hypoxia, cardiomyopathy, acute renal failure)
Known or anticipated blood withdrawal within 6 weeks that exceeds the NIH guidelines of 450 l/six weeks in adults or 7 ml/kg/six weeks in children.
BONE MARROW TRANSPLANT COMPONENT:
INCLUSION CRITERIA:
Participation in a Clinical Center bone marrow transplant protocol for breast cancer (generally Allogeneic Breast Protocol 2: Phase 1 Trial of T cell Exchange with Th2/Tc2 Cells for Allogeneic Stem Cell Transplantation after Reduced Intensity Conditioning for Metastatic Breast Cancer), or for hematologic malignancy (generally either: T-cell depleted, reduced intensity allogeneic stem cell transplant from haploidentical related donors for hematologic malignancies: A sequential dose escalation study of donor Th2/Tc2 cells or Th2, Sirolimus in Allogeneic HSCT.
Agreement from the oncologist PI that the patient may participate in this protocol.
EXCLUSION CRITERIA:
The presence of any contraindication to insulin tolerance testing-e.g.cardiovascular or cerebrovascular disease or any seizure history.
Pregnancy
Age less than 15 years
End stage renal or liver disease as defined under the critical care section
Known or anticipated blood withdrawal within 6 weeks that exceeds the NIH guidelines of 450 ml/six weeks in adults.
HEALTHY VOLUNTEER COMPONENT:(Closed to recruitment)
INCLUSION CRITERIA:
Adults aged at least 18 years will be recruited.
EXCLUSION CRITERIA:
Severe hepatic, renal, cardiac, psychiatric or neurological illnesses
More than two weeks of oral antifungal or glucocorticoid medications or near daily use of topical glucocorticoids with broken skin. Frequent use of topical antifungal agents will be considered on a case-by-case basis.
Pregnancy
KNOWN ADRENAL INSUFFICIENCY COMPONENT:
INCLUSION CRITERIA:
Documented longstanding primary or secondary adrenal insufficiency
EXCLUSION CRITERIA:
Pregnancy
TRANSIENT ADRENAL INSUFFICIENCY COMPONENT:
INCLUSION CRITERIA:
Recent uncomplicated successful transsphemoidal surgery for Cushing s disease with serum cortisol level less than 5 micro g/dl
TRANSIENT ADRENAL INSUFFICIENCY COMPONENT:
EXCLUSION CRITERIA:
Supra physiologic dosing of glucocorticoids as a treatment for another underlying medical disorder or surgical complication
Pregnancy
CIRRHOSIS AND NEPHROTIC SYNDROME COMPONENT:
Adults aged at least 18 years will be recruited.
CIRRHOSIS
INCLUSION CRITERIA:
* Participation in an active NIDDK protocol entitled: Evaluation of patients with liver disease (91-DK-0214), with evidence of chronic liver disease
* Agreement from the hepatologist PI that the patient may participate in this protocol.
* Child-Pugh class A or B cirrhosis secondary to viral hepatitis
* Absence of other significant medical illnesses that might interfere with prolonged follow-up evaluation
* Normal renal function (creatinine clearance estimated as \> 60 ml/min by the Modified Diet in Renal Disease (MDRD) equation and reported through our Clinical Research Information System (CRIS)).
EXCLUSION CRITERIA:
* History of the use of glucocorticoid therapy or medications that are known to interfere with HPA axis function within the past 6 months
* Current symptoms of adrenal insufficiency (nausea, vomiting, weight loss, lightheadedness, unusual fatigue, salt craving, etc.).
* Evidence of moderate-severe medical illness attributable to obstructive sleep apnea, heart or pulmonary failure, or active malignancy will be excluded.
* Pregnancy
* Known or anticipated blood withdrawal within 6 weeks that exceeds the NIH guidelines of 450 ml/six weeks in adults.
NEPHROTIC SYNDROME:
INCLUSION CRITERIA:
* Participation in one of three active NIDDK protocols entitled: Rituximab plus cyclosporine in idiopathic membranous nephropathy (09-DK-0223), the Nephrotic Syndrome Study Network (Neptune) (11-DK-0023), or Pathogenesis of Glomerulosclerosis Study (94-DK-0127) with previously documented proteinuria \>3.5g/day for 2 months or more or protein/creatinine ratio of 2.0 g/g on at least 2 occasions
* Agreement from the nephrologist PI that the patient may participate in this protocol. Estimated GFR greater than or equal to 30 ml/min based on the MDRD equation and reported through CRIS.
* Normal liver function as defined by normal liver function tests and no known history of liver disease.
EXCLUSION CRITERIA:
* History of the use of glucocorticoid therapy or medications that are known to interfere with HPA axis function within the past 6 months
* Current symptoms of adrenal insufficiency (nausea, vomiting, weight loss, lightheadedness, unusual fatigue, salt craving, etc.).
* Evidence of moderate-severe medical illness attributable to obstructive sleep apnea, heart or pulmonary failure, or active malignancy will be excluded.
* Pregnancy
* Known or anticipated blood withdrawal within 6 weeks that exceeds the NIH guidelines of 450 ml/six weeks in adults.
