Clinical Research Directory

* INCLUSION CRITERIA: 1. Age 18 to 65 years, inclusive (males and females) 2. Able to travel to the NIEHS CRU for required study visits 3. Able to present a valid government-issued form of identification for entry to the NIEHS campus 4. Able to fast for 8 hours prior to the visit where bronchoscopy occurs 5. Has a responsible party who is willing and able to attend Visit 2 and drive the participant away from the CRU after completion of Visit 2, if the participant elects to undergo conscious sedation 6. Genotype information available for relevant CYP2J2 and EPHX2 polymorphisms, which indicates: * Wild type with respect to EPHX2 K55R, EPHX2 R287Q, and CYP2J2\*7; or * Homozygous for EPHX2 K55R (wild type for the other SNPs); or * Homozygous for EPHX2 R287Q (wild type for the other SNPs); or * Homozygous for CYP2J2\*7 (wild type for the other SNPs) EXCLUSION CRITERIA: 1. Current pregnancy or lactation, as medications used during the bronchoscopy can be excreted in the breast milk of lactating mothers 2. Current smoker or significant second-hand smoke exposure (defined by urine cotinine \>200 ng/mL at screening) 3. Post-bronchodilator FEV1 \< 70% of predicted 4. For asthmatics, any indication of moderate or severe asthma such as: * Physician-directed emergency treatment for an asthma exacerbation within the preceding 12 months * Any use of systemic steroid therapy during the last year or continuous use of inhaled steroids over a period of 1 month or longer during the past 6 months * Regular use of cromolyn (except for prophylaxis of exercise induced bronchospasm) or any use of leukotriene inhibitors (Montelukast or Zafirlukast) within the past month * Symptoms including: * Night-time symptoms of cough or wheeze greater than 1x/week * Exacerbations of asthma more than 2x/week * Daily requirement for albuterol due to asthma symptoms (cough, wheeze, chest tightness) 5. Bleeding disorders or regular use of aspirin or other non-steroidal anti-inflammatory drugs (which inhibit platelet function) or other drugs that prolong bleeding time such as warfarin, heparin or derivatives, or clopidogrel and related ADP inhibitors 6. Sickle cell disease or GP6 deficiency 7. Facial deformity or major facial surgery 8. Asthma exacerbation or respiratory infection 4 weeks prior to study visit 9. Allergy or history of adverse reactions to lidocaine, midazolam, or fentanyl 10. Vital signs (temperature, blood pressure, pulse rate) that are outside of the established CRU limits 11. Body weight \<50 kg (\<110 lbs) 12. The following abnormal clinical laboratory values (obtained during Visit 1 assessment): * Platelet count \<100,000 per L * White blood cells count \<3000 per L * Absolute neutrophil count \<1000 per L * Hematocrit \<35% for both female and male * PT/INR and PTT based on reference laboratory established reference ranges * Serum creatinine \>1.4 mg/dL 13. Any condition that, in the investigator's opinion, places the participant at undue risk for complications associated with required study procedures. 14. Use of the medication colchicine (commonly used to treat gout attacks) in the past 2 weeks. Volunteers meeting any of the below temporary exclusions may enroll in the study, but may not undergo the bronchoscopy procedure within the time frames specified for each below temporary exclusion. If any of the following conditions are met, the bronchoscopy will be rescheduled such that none of these temporary exclusions apply: Temp. EXCL 1. An asthma exacerbation requiring increased asthma medications for more than 1 day (and other than exercise-induced asthma) within 1 month of bronchoscopy Temp. EXCL 2. Viral upper respiratory tract infection or any acute infection requiring antibiotics within 4 weeks of bronchoscopy Temp. EXCL 3. Use of anti-inflammatory medications (including over-the-counter preparations) during the 48 hours prior to the bronchoscopy and aspirin during the 14 days prior to bronchoscopy. Temp. EXCL 4. Any food or fluids for 8 hours prior to the bronchoscopy