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Diagnosis of Tuberculosis in Swiss Children
Sponsor: University Children's Hospital Basel
Summary
1. The primary objective is to improve the sensitivity of novel immunodiagnostic tests for detection of TB disease in children. 2. The secondary objective is to determine biomarkers that discriminate children with TB infection and disease.
Official title: Evaluation and Validation of Novel Immunodiagnostic Tests for Childhood Tuberculosis Infection and Disease in Switzerland. The CITRUS (ChIldhood TubeRcUlosis in Switzerland) Study.
Key Details
Gender
All
Age Range
Any - 17 Years
Study Type
OBSERVATIONAL
Enrollment
190
Start Date
2017-05-12
Completion Date
2028-12
Last Updated
2024-09-19
Healthy Volunteers
No
Conditions
Interventions
Secreted cytokine assay
Blood collected in a sodium-heparin tube will be used for the assay using novel TB-specific antigens. After incubation the supernatant will be harvested and cryopreserved at -80 °C for further testing of cytokines. Cytokines will be measured using a bead-based multiplex assay reader (MAGPIX, Luminex Crop., Austin, USA).
Intracellular cytokine assay
Blood will be stimulated with recombinant MTB-specific antigens, ESAT-6, CFP-10, positive control or left unstimulated. Following an initial duration of stimulation, Brefeldin-A will be added and the blood incubated for a further 5 hours. White cells will be fixed and cryopreserved at - 80°. Batched analysis within 6 months of cryopreservation will be done using multi-colour flow cytometry.
Locations (8)
Kantonspital Aarau
Aarau, Switzerland
Nina Vaezipour
Basel, Switzerland
Ospedale Regionale di Bellinzona
Bellinzona, Switzerland
Inselspital Bern
Bern, Switzerland
Hôpital des enfants - HUG
Geneva, Switzerland
Kinserspital Luzern
Lucerne, Switzerland
Kinderspital St Gallen
Sankt Gallen, Switzerland
Kinderklinik Zürich
Zurich, Switzerland