Clinical Research Directory

Inclusion Criteria: * Cohort 1: healthy un-medicated males (to limit gender-induced variability similar to our pilot study), shift-work schedule (\>3 shifts per month outside 7am-6pm (9)) for the past ≥10 years, 40-59 years old (increased prevalence of the metabolic syndrome at ≥60 years of age (20)); * Cohort 2: day workers who work 7am-6pm for ≥10 years matched for line of work, age, gender, and BMI; * Volunteers are capable of giving informed consent; * 40-59 years of age; * Own an android smartphone which installs the remote sensing applications (those with apple smartphones will not be recruited); * Non-smoking; * Male subjects * The use of contraception will NOT be required for male participants. Exclusion Criteria: * Recent travel across more than two (2) time zones (within the past month); * Planned travel across more than two (2) time zones during the planned study activities; * Use of illicit drugs; * High dose vitamins (Vitamin A, Vitamin C, Vitamin E, Beta Carotene, Folic Acid and Selenium), alcohol and any over-the counter NSAID in the (2) two weeks before the start of the 48 hour deep phenotyping; * High fat foods and caffeine in the past 24 hours prior to the 48-hour deep chronotyping session; * History of abdominal surgery; * Known allergy or intolerance to Vancomycin, and/or Neomycin; * Use of anticholinergics in the week prior to the 48-hour sessions; * Use of laxatives or anti-diarrhea medications in the two weeks prior to the 48-hour sessions; * Subjects, who have received an experimental drug, used an experimental medical device within 30 days prior to screening, or who gave a blood donation of ≥ one pint within 8 weeks prior to screening; * Subjects with any abnormal laboratory value or physical finding that according to the investigator may interfere with interpretation of the study results, be indicative of an underlying disease state, or compromise the safety of a potential subject;