Clinical Research Directory
Browse clinical research sites, groups, and studies.
Physiologic and Functional Adaptations of Insulin Sensitive Tissues
Sponsor: Vanderbilt University Medical Center
Summary
The long-term goal is to understand the mechanisms of intestinal nutrient sensing and signal relays to insulin sensitive tissues (adipose, skeletal muscle, liver) in humans. The investigators hypothesize that human tissue biopsies (from obese surgery and non-surgery subjects as well as lean controls) can be used to understand the molecular mechanisms underlying intestinal nutrient sensing and signal relay in humans. The investigator will obtain tissue specimens from patients during scheduled upper endoscopies, colonoscopies and scheduled metabolic and bariatric surgeries or liver transplantation. A blood sample (4mL) will be obtained concurrent with these procedures. From metabolic and bariatric surgery subjects blood and tissues (liver, adipose, small intestine, omentum, skeletal muscle) can be collected at the time of surgery. From liver transplantation patients, excised liver tissue will be collected. Stool can be obtained preoperatively and at various time points after surgery. Some bariatric surgery subjects will participate in a mixed-meal tolerance test at their pre-operative visit and several post-operative visits to compare the whole-body metabolic alterations following bariatric procedures.
Official title: Physiologic and Functional Adaptations of Insulin Sensitive Tissues After Bariatric Surgery
Key Details
Gender
All
Age Range
18 Years - Any
Study Type
OBSERVATIONAL
Enrollment
500
Start Date
2018-02-13
Completion Date
2028-02
Last Updated
2025-12-18
Healthy Volunteers
No
Conditions
Interventions
Endoscopy biopsy
Tissue samples will be collected from the jejunum, duodenum, and colon during the subject's standard of care procedure.
Surgical biopsy
Biopsy of the liver is standard of care for diagnosing NAFLD. Biopsy of omentum, skeletal muscle and subcutaneous adipose (the latter two tissues taken at the site of trocar placement) will be collected intraoperatively at the time of Roux-en-Y gastric bypass (RYGB) or vertical sleeve gastrectomy (VSG).
Mixed-meal tolerance test
The mixed meal tolerance test (MMTT) shows how much insulin your body is making after drinking a liquid meal beverage that contains fats, protein and carbohydrates (sugar). Glucose, C-peptide, free fatty acids, triglycerides, incretins, pancreatic polypertide, bile aceis, and fatty acid amides can also be measured. A total of 9 tablespoons of blood is taken over a 255 minute period.
Excised Liver Tissue Sampling
Tissue will be collected from the excised liver for research after the diseased liver has been removed as part of the standard of care procedure.
Locations (1)
Vanderbilt University Medical Center
Nashville, Tennessee, United States