Clinical Research Directory

Inclusion Criteria: * female * 55 years of age and above * any ethnicity or race * screened to sit for 8 or more hours per day, perform less than 60 sit-to-stand transitions per day \& engage in less than 20 minutes of moderate-to-vigorous physical activity (MVPA) per day * BMI 25 - 45 kg/m2 * ambulatory * medically stable without any health conditions that would inhibit standing or PA; * able to give informed consent \& comply with study protocols; * able to read, communicate, and write fluently in English; * able to travel to study visits; * no menstruation for at least one year; * not participating or not planning to participate in a physical activity or weight loss program or research study. Exclusion Criteria: * Mental state that would preclude complete understanding of the protocol or compliance; * type 1 diabetes; * type 2 diabetes using insulin or with poor glycemic control (greater than 10% for all participants); * poorly controlled hypertension (Systolic Blood Pressure ≥165 or Diastolic Blood Pressure ≥100); * chronic illness that may be associated with weight change (HIV/AIDS, active cancer, or uncontrolled thyroid disease), * anemia (hemoglobin ≤11g/dL); * personal or first-degree relative history of venous thrombosis; * weight instability in past 3 months (no more than 5% up or down); * regular use of vasodilator medication and high risk of stroke and/or heart attack (i.e., history of multiple hospitalizations (\>2X) in the last 6 months), congestive heart failure, atrial fibrillation, and/or stroke; * regular use of immunosuppressant or corticosteroid medication; * blood donation less than 56 days prior to screening visit, * smoking cigarettes or smoking or ingesting anything, and other use of tobacco products including e-cigarettes. * diagnosis of orthostatic hypotension