Clinical Research Directory

Inclusion Criteria: * written informed consent from all participants before the study * a current medical clearance to practice sports, * training experience: at least 1 year strength training programs that include lower limbs, * minimum of 3 workout sessions (strength/resistance training) a week, * protein intake in customary diet and during the study protocol \> 1.6g / kg of body mass per day, * individual maximum strength greater than 2.5 times the body weight for the 1RM test in the leg-press. Exclusion Criteria: * current injury, * any health-related contraindication, * declared general feeling of being unwell, * unwilling to follow the study protocol, * serious disease or metabolic problems, * intake of creatine, caffeine or beta-alanine, or other ergogenic supplements 3 months before the beginning of the study, * history of anabolic androgenic steroids or drugs use that may interfere with muscle mass control (eg, corticosteroids), or affect physical performance, * smoking and tobacco use, * presence of infectious disease in the previous 4 weeks of the study.