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Pumilio1 (PUM1) Expression, Sickle Cell Anemia, β-thalassemia Intermedia
Sponsor: Assiut University
Summary
1. To study the expression pattern of PUM1 gene in patients with sickle cell anemia and β-thalassemia intermedia. 2. To detect PUM1 protein levels in sickle cell anemia and β-thalassemia intermedia patients. 3. To correlate PUM1 gene expression pattern and protein levels with HbF levels in sickle cell anemia and β-thalassemia intermedia patients.
Official title: Detection of RNA Binding Protein Pumilio1 (PUM1) Expression in Patients With Sickle Cell Anemia and β-thalassemia Intermedia
Key Details
Gender
All
Age Range
Any - Any
Study Type
OBSERVATIONAL
Enrollment
80
Start Date
2023-08
Completion Date
2027-12
Last Updated
2023-05-31
Healthy Volunteers
Not specified
Interventions
RNA Binding Protein Pumilio1 (PUM1) Expression by reverse transcriptase quantatative PCR
Quantatative Real -Time PCR for quantification of PUM1; * The type of the recruited samples: Ethylenediamine tetra-acetic acid (EDTA) peripheral blood samples. * The method of total RNA extraction: TRIzol and TRIzol LS. * The purity and concentration of the RNA were measured using a Nano Drop 2000 instrument. * cDNA was amplified with primers using the GoScript Reverse Transcription system. Western Blotting Assay of PUM1 protein levels