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Fetal Cell Receptors Repertoire
Sponsor: Assistance Publique - Hôpitaux de Paris
Summary
The purpose of this study is to describe the transcriptomic profile of foetal cells in post-partum and more specifically to determine which chemokine receptors are overexpressed in foetal cells in post-partum women with wounds To do so, the investigators will isolate foetal cells from the peripheral blood of healthy controls post partum women as well as from post partum women with skin ulcers and then perform RNA sequencing.
Official title: "Study of CCR Receptor Overexpression in Fetal Microchimeric Cells: Proof of Concept Before a Potential Clinical Trial"
Key Details
Gender
All
Age Range
Any - Any
Study Type
INTERVENTIONAL
Enrollment
160
Start Date
2024-02-06
Completion Date
2027-02-01
Last Updated
2025-09-12
Healthy Volunteers
Yes
Interventions
Saliva sampling
HLA genotyping. The technique should allow to identify, for children's, a paternal HLA antigen not shared with the mothers.
Blood sampling
Maternal Blood samples will be incubated with the appropriate antibody, targeting the microchimeric fetal cells of each patient, as well as with a cell viability marker (DAPI). The samples were then be processed through the BD FACS Aria III to sort the fetal cells, The following steps - RNA extraction, quality control, retrotranscription, preparation of the library, sequencing and transcriptomic analysis - will be carried out according to the Smart-seq3 protocol. The data will be sent for in-depth analysis and confirmation of the results. Additional functional experiments may also be carried out.
Interviews
V2 and/or V3
Clinical examination
V2 and/or V3
Locations (1)
Dermatology unit - Cochin Hospital - APHP
Paris, Île-de-France Region, France