Inclusion Criteria:
1. Age ≥ 18 years.
2. Histopathologically proven newly diagnosed primary glioblastoma.
3. Patients must have tumours which are MGMT-unmethylated.
4. Patients must be CMV-seropositive.
5. The tumour must be supratentorial.
6. Received debulking surgery. This includes complete or partial surgical resection. Biopsy alone is not acceptable.
7. Will have undergone standard concurrent radiotherapy (XRT) and temozolomide (TMZ) by the time of stage 2 participation consent.
8. Patients who are being treated with stable or decreasing doses of dexamethasone (\>/= 4 mg/day) or other steroid equivalent, at the time of post-XRT adjuvant TMZ initiation.
9. Brain MRI (gadolinium-enhanced) within 31 days prior to the adjuvant TMZ.
10. Patients with neurological deficits should have deficits that are stable for a minimum of 2 weeks prior to stage 2 participant consent and should remain stable prior to the commencement of adjuvant TMZ.
11. ECOG 0-2 if \>/= 70 years. ECOG 0-1 if aged \> 70 years.
12. Life expectancy of \> 12 weeks.
13. Adequate bone marrow function:
* platelet count ≥ 100 x 109/L
* absolute neutrophil count (ANC) ≥ 1.5 x 109/L
* Haemoglobin ≥ 10g/dl.
14. Adequate liver function:
* (alanine transaminase (ALT)/aspartate transaminase (AST) ≤ 3.0 times the upper limit of normal (ULN)).
* Total bilirubin ≤ 1.5 x ULN (Exception: Patient has known Gilbert's Syndrome or is suspected of having it, for which additional lab testing of direct and/or indirect bilirubin supports this diagnosis. In these instances, a total bilirubin of ≤ 3.0 x ULN is acceptable).
15. Adequate renal function:
• creatinine ≤ 1.5 x ULN
16. Willing to comply with all study requirements, including treatment, timing and/or nature of required assessments
17. Signed, written informed consent.
Exclusion Criteria:
1. Pregnant or need to breastfeed during the study period (Negative serum pregnancy test required for women of childbearing potential). Not adhering to pregnancy prevention recommendations.
2. Active infection requiring treatment or an unexplained febrile (\> 39 C) illness within a week of starting the trial.
3. Patients with previous inguinal lymph node dissection, radiosurgery, brachytherapy, or radiolabelled monoclonal antibodies.
4. Prior allogeneic solid organ transplant. Currently receiving or ever received immunosuppressive therapy for an organ transplant.
5. Active or prior documented autoimmune or immunosuppressive disease that has required systemic treatment (i.e. with the use of disease-modifying agents, corticosteroids, or immunosuppressive drugs). Replacement therapy such as thyroxine, insulin, or physiologic corticosteroid replacement therapy for adrenal or pituitary insufficiency is allowed. Use of non-systemic topical steroids is allowed.
6. Patients receiving immunosuppressive medication or prior use within 14 days before the first dose of investigational product are excluded, except for systemic steroids up to 4 mg dexamethasone per day for the management of CNS symptoms.
7. Patients on bevacizumab or who have received prior bevacizumab as management for their GBM
8. Known human immunodeficiency virus infection and/or hepatitis B or C infections OR known to be positive for hepatitis B antigen (HBsAg)/Hepatitis B virus (HBV) DNA or Hepatitis C antibody or RNA.
9. Patients with poorly controlled, unstable or severe intercurrent medical conditions such as renal, cardiac (congestive cardiac failure, myocardial infarction within 6 months, myocarditis), or pulmonary disease or other condition, therapy or laboratory abnormality that might confound the results of the study, interfere with the patient's participation, make administration of the study drug hazardous or make it difficult to monitor adverse effects, in the opinion of the treating physician.
10. Prior conventional antitumour therapy, other than steroids, RT or TMZ therapy given for glioblastoma.
11. Allergic or unable to tolerate TMZ for any reason. Any patient that successfully completed at least 5 weeks of TMZ during standard of care XRT/TMZ and whose blood counts meet the eligibility requirements (inclusion #13) within 6 weeks post XRT/TMZ is eligible.
12. Patients receiving any other investigational drug therapy or having participated in an investigational drug/device study within 4 weeks prior to the first dose of study treatment.
13. Specific comorbidities or conditions (e.g. psychiatric) or concomitant medications which may impact the administration of study-related treatments or procedures.
14. Radiographic or cytologic evidence of leptomeningeal or multifocal disease at any time prior to treatment.
15. History of another malignancy within 2 years prior to registration. Patients with a history of adequately treated carcinoma-in-situ, basal cell carcinoma of the skin, squamous cell carcinoma of the skin, or superficial transitional cell carcinoma of the bladder are eligible. Patients with a history of other malignancies are eligible if they have been continuously disease-free for at least 2 years after definitive primary treatment.
16. Receipt of other live and attenuated vaccines within 30 days of treatment product will be excluded.
17. Unresolved toxicities from prior anticancer therapy, defined as not having resolved to NCI CTCAE grade 0 or 1, with the exception of alopecia.
18. Patients with a prior severe hypersensitivity reaction to treatment with any monoclonal antibody and/or components of the study vaccine.
19. Patients with any known severe allergies to gadolinium contrast agents.
