Clinical Research Directory

Inclusion Criteria: Participants must meet all of the following inclusion criteria to be enrolled in the study: 1. Age 18-70 years old (including 18 years old and 70 years old), regardless of gender; 2. Can understand the study and have signed the informed consent; 3. Expected survival time \> 3 months; 4. The American Eastern Oncology Consortium (ECOG) score was 0-1; 5. Cd20-positive B-NHL was confirmed cytologically or histologically according to WHO 2016 criteria These include diffuse large B-cell lymphomas (including histologically transformed forms) and transformed follicular lymphomas (TFL); (For the expression status of CD20, the histological diagnosis of CD20 has been clearly documented in the past Positive subjects (diagnosis within 3 months prior to screening); Subjects without prior records, Pathological specimens provided or collected by our hospital were diagnosed as CD20 positive; Not without a clear record For those who provide or collect specimens, the researchers and sponsors will decide whether to be included according to their medical records); 6. For recurrent or refractory B-cell non-Hodgkin lymphoma, subjects must have at least been treated with anthracene Treatment with cyclodrugs and rituximab (or other CD20-targeting drugs), and have already received them At least two cycles of treatment; Refractory is defined as the best response to the most recent treatment regimen as disease progression, or the last treatment The optimal response of the regimen (at least 2 cycles) was stable disease with a duration of less than 6 months. 7. Does not meet the criteria for autologous hematopoietic stem cell transplantation (auto-HSCT) or is unwilling to perform autologous transplantation Patients with recurrence or progression after blood stem cell transplantation or autologous hematopoietic stem cell transplantation; 8. The venous access required for mononuclear cell collection can be established, and the hemoglobin is ≥70 g/L and neutral Granulocyte ≥ 1.0×109/L, platelet ≥50×109/L, a single nucleus can be performed by the investigator's judgment Cell collection; 9. Evaluable lesions identified according to the 2014 Lugano efficacy evaluation criteria; 10. Organ functions meet the following requirements: 1. The investigator assessed sufficient bone marrow function to receive lymphocyte clearance chemotherapy; 2. Serum creatinine ≤1.5× upper limit of normal (ULN) or creatinine clearance (Cockcroft and Gault) \> 30 mL/min/1.73 m2; 3. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) ≤3× ULN (ALT and AST in patients with liver metastasis ≤5×ULN); 4. Total bilirubin ≤2.0 × ULN (for patients with Gilbert's syndrome or lymphoma invading the liver≤3 × ULN); 5. Lung function: ≤CTCAE grade 1 dyspnea and oxygen saturation of basic finger pulse in indoor air environment Sum degree ≥92%; 6. Cardiac function: Normal diastolic function, echocardiography or radiation within 1 month prior to enrollment Sexual nuclide active angiography (MUGA) revealed left ventricular ejection fraction (LVEF) ≥ 50%, no clinically significant pericardial effusion. Exclusion criteria Subjects who meet any of the following criteria will not be enrolled in this study Exclusion Criteria: Subjects who meet any of the following criteria are excluded from the study: 1. Fully treated cervical carcinoma in situ and a history of other malignant tumors within 5 years prior to screening Adeno-papillary carcinoma, basal cell or squamous cell skin cancer, local front line after radical surgery Except adenocarcinoma and ductal carcinoma in situ after radical surgery; 2. Hepatitis B surface antigen (HBsAg) positive, or Hepatitis B core antibody (HBcAb) positive and peripheral The blood HBV DNA titer was higher than the lower limit. Hepatitis C virus (HCV) antibody positive and HCV RNA titer was higher than the lower limit of detection. Positive for human immunodeficiency virus (HIV) antibodies; Serological test positive for syphilis; 3. Any of the following unstable diseases (including but not limited to) have occurred in the 6 months prior to screening Stable angina pectoris; Cerebral ischemia or cerebral vascular accident; Myocardial infarction; Congestive heart failure (New York Heart Association \[NYHA\] classification ≥III); Severe cardiac arrhythmia requiring medical treatment Often; After heart angioplasty or coronary stent implantation or heart bypass surgery; mining History of deep vein thrombosis or pulmonary embolism within 6 months prior to cell collection; 4. Lymphoma involving the central nervous system (CNS) only (subjects with secondary CNS lymphoma) Allow to be included); 5. Previous or clinically significant central nervous system history or disease at the time of screening, such as epilepsy, epilepsy Seizures, paralysis, aphasia, stroke, severe brain injury, dementia, Parkinson's disease, cerebellar disease, Organic syndrome or mental illness; 6. Active autoimmune disease (including but not limited to systemic lupus erythematosus, dry disease) within 2 years Dryness syndrome, rheumatoid arthritis, psoriasis, multiple sclerosis, inflammatory bowel disease, Hashimoto Thyroiditis, etc., except for hypothyroidism that can only be controlled by hormone replacement therapy); 7. Active or uncontrolled infection requiring systemic treatment within 14 days prior to apheresis; 8. There are active tuberculosis patients in the screening period; 9. During the screening period, there are lymphoma lesions in the lung or gastrointestinal tract that are considered by investigators to be at risk of bleeding Of patients; 10. Was receiving systemic steroid therapy prior to screening and was determined by the investigator to be prolonged during the study period Subjects treated with systemic steroids; Systemic treatment was used within 72 hours before cell transfusion Alcohol-treated subjects (except for inhalation or topical use); 11. Pre-amperectomy CAR-T therapy (except previous CD19-targeted CAR-T therapy) Efficacy was evaluated as disease stable or at any time at 3 months after CD19 CAR-T reinfusion Efficacy evaluation for patients with disease progression) or other genetically modified T cell therapy; 12. Received autologous stem cell transplantation within 6 weeks before anapheresis; 13. Presence of grade ≥2 acute graft-versus-host disease (GVHD) requiring treatment within 4 weeks prior to anaphermology (Glucksberg standard) or generalized chronic GVHD (Seattle Standard) or by researchers Who may need anti-GVHD therapy during the trial; 14. Treatment with alenzumab 6 months before monocyst, or fludarabine or Fludarabine within 3 months before monocyst Cladobine treatment; 15. Received non-lymphocytotoxic cytotoxic chemotherapeutic drugs within 1 week before anapheresis. If it's a single harvest If the former oral chemotherapy drug has passed at least 3 half-lives, it is allowed to be included in the group. 16. Received lymphocytotoxic chemotherapy (e.g. Cyclophosphamide, IFosfamide, benzene) within 2 weeks of preharvest Damustine); 17. Use of other study drugs within 4 weeks prior to anapheresis. But ineffective or disease progression during the trial, and If at least 3 half-lives had passed before the single harvest, they were allowed to be included. 18. LY007 cell injection received donor lymphocyte infusion (DLI) within 6 weeks prior to administration; 19. Received radiotherapy within 6 weeks prior to anapheresis. Disease progression at the subject's radiotherapy site, or other unirradiated areas Only patients with PET positive lesions were eligible for inclusion. If PET is present in other unirradiated sites For positive lesions, radiotherapy for a single lesion was allowed 2 weeks before anapheresis. 20. Acute side effects caused by previous treatment did not recover to grade 1 or below (hematological toxicity and alopecia) Except); 21. The presence of life-threatening hypersensitivity or other intolerance to the drugs used in the study Severe allergies; 22. Any of the following: 1. Platelet transfusion within 1 week before enrollment; 2. Red blood cell (RBC) transfusion within 2 weeks prior to enrollment; 3. Granulocyte macrophage colony-stimulating factor (GM-CSF) was administered during the first 2 weeks of enrollment. 23. Persons who are pregnant or breastfeeding, or who plan to become pregnant within 1 year after cell transfusion, or partners in their cell cycle Male subjects planning pregnancy within 1 year after transfusion; 24. Any situation that the investigator believes will compromise the safety of the subject or interfere with the purpose of the study or cannot be complied with Adhere to the study protocol or other circumstances that are not suitable for inclusion