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Autoantibodies Against-nephrin in Idiopathic Nephrotic Syndrome
Sponsor: Mario Negri Institute for Pharmacological Research
Summary
This retrospective study is aimed at evaluating the levels of circulating anti-nephrin autoantibodies in patients with INS, including those with MCD/FSGS and in patients who have experienced relapse of FSGS post-transplant, compared to those of a control group of patients with nephrotic syndrome due to primary membranous nephropathy (MN).
Key Details
Gender
All
Age Range
18 Years - Any
Study Type
OBSERVATIONAL
Enrollment
100
Start Date
2024-03-19
Completion Date
2028-03
Last Updated
2025-09-04
Healthy Volunteers
No
Conditions
Interventions
In-house ELISA, and ELISA kits from "DBA Italy" (Abbexa).
"Nunc MaxiSorp" ELISA plates will be coated with recombinant extracellular domain of human nephrin. Patient serum samples will be added in appropriate dilution. Plates will be then incubated with biotin-conjugated anti human IgG antibody followed by incubation with horseradish peroxidase (HRP)-avidin conjugate. Then tetramethylbenzidine substrate will be added, and absorbance read at 450 nm.
Locations (1)
Clinical Research Center for Rare Disease Aldo e Cele Daccò
Ranica, BG, Italy