Inclusion Criteria:
* Inclusion criteria for healthy volunteers:
1. 18 years old ≤ age ≤75 years old, gender is not limited;
2. No inflammatory bowel disease, lactose intolerance, intestinal bacterial overgrowth, parasitic infection, drug induced enteritis and other intestinal diseases;
3. No abdominal pain, frequency of defecation and changes in fecal characteristics in 1 week;
4. Provide healthy subjects with no obvious abnormalities reported by colonoscopy in the past 1 year;
5. Healthy subjects who can provide medical examination reports within the past 1 year and have routine physical examination conducted by researchers to confirm that there are no heart and lung diseases, digestive, urinary, blood, endocrine, nervous system and other serious underlying diseases;
6. No antidiarrheal drugs, antibiotics, gastrointestinal motility drugs or other related drugs affecting clinical studies within 4 weeks;
7. Clear consciousness, can complete normal communication;
8. Understands and is willing to comply with the research protocol and sign the informed consent.
* Inclusion criteria for IBS patients:
1. Patients who can provide colonoscopy report in the past 1 year and meet the Rome IV standard of irritable bowel syndrome and TCM syndrome differentiation type;
2. Provide medical examination reports within the past 1 year to confirm patients without digestive, circulatory, respiratory, urinary, reproductive, endocrine, nervous system and other malignant tumors and serious underlying diseases;
3. 18 years old ≤ age ≤75 years old, gender is not limited;
4. No antidiarrheal drugs, antibiotics, gastrointestinal motility drugs or other related drugs that affect clinical studies have been taken within 4 weeks;
5. Clear consciousness, can complete normal communication;
6. Understands and is willing to comply with the research protocol and sign the informed consent.
Exclusion Criteria:
* Exclusion criteria for healthy volunteers:
1. Those who have mental illness, alcohol dependence or a history of drug abuse and cognitive impairment are unable to cooperate with the examination and understanding of the subject;
2. Female volunteers who are pregnant or lactating, or in ovulation and menstruation period;
3. Volunteers with current abnormal body temperature;
4. Subjects currently participating in other clinical trials.
* Exclusion criteria for patients with IBS:
1. Those who have mental illness, alcohol dependence or a history of drug abuse and cognitive impairment are unable to cooperate with the examination and understanding of the subject;
2. Pregnant or lactating women or female subjects in ovulation and menstruation period;
3. Patients with inflammatory bowel disease, lactose intolerance, intestinal bacterial overgrowth, parasitic infections, drug-induced enteritis and other intestinal diseases;
4. Patients with intestinal diseases such as functional constipation, functional diarrhea, opioid-induced constipation, and non-specific functional bowel disease;
5. Patients with functional gastrointestinal diseases such as functional dyspepsia, functional heartburn, central abdominal pain syndrome;
6. Patients with current abnormal body temperature;
7. Participants who are currently participating in other clinical trials.
