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NCT06440603

EKLF Gene Expression in β-thalassemia

Sponsor: Rofaida Hassan Ahmed

View on ClinicalTrials.gov

Summary

1. Studying the effect of expression pattern of EKLF gene in β-thalassemic patients. 2. Detecting the correlation between the gene expression of EKLF and the clinical phenotype of β-thalassemic patients.

Official title: Erythroid Krüppel Like Factor (EKLF) Gene Expression in β-thalassemia Patients

Key Details

Gender

All

Age Range

5 Years - 80 Years

Study Type

OBSERVATIONAL

Enrollment

150

Start Date

2024-07-01

Completion Date

2027-07-01

Last Updated

2024-06-04

Healthy Volunteers

Yes

Conditions

β-thalassemia

Interventions

DIAGNOSTIC_TEST

PCR

Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) for detection of EKLF: * The method of total RNA extraction: TRIZOL and TRIZOL LS. * The purity and concentration of the RNA will be measured using Nano Drop 2000 instument. * cDNA will be done with primers using thr Goscript Reverse Transcription System