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Antimicrobial Efficacy of Nano-Based ICM on E.F
Sponsor: Tanta University
Summary
This in-vivo study aims to evaluate the antimicrobial effect of nano based intracanal medications (Triple antibiotic-loaded chitosan nanoparticles and chlorohexidine loaded by silver nanoparticles) on Enterococcus faecalis count reduction in secondary endodontic infection cases.
Official title: Antimicrobial Efficacy of Nano-Based Intracanal Medications on Enterococcus Faecalis
Key Details
Gender
All
Age Range
19 Years - 60 Years
Study Type
INTERVENTIONAL
Enrollment
30
Start Date
2024-01-02
Completion Date
2025-03-02
Last Updated
2024-08-01
Healthy Volunteers
Yes
Interventions
antimicrobial efficacy of nano based intracanal medications on Enterococcus faecalis count reduction in secondary endodontic infection cases.
The paper points of three samples (S1, S2, and S3) will be placed each in a sterile falcon tube containing 1ml of brain heart infusion and labeled according to the type of intracanal medication used. Samples will be homogenized by the vortex and and one micron of each sample will be cultured on Bile Esculin Agar for E. faecalis. The plate will be labeled as the tube of the same sample and incubated at 37οC for 24-48 h and then after incubation, the total numbers of E.faecalis will be counted and calculated to get the colony forming units (CFU/ml).
Locations (1)
Faculty of Dentistry
Tanta, Egypt