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Hepatic Mitochondrial Metabolism in Fatty Liver Disease in Humans
Sponsor: Helsinki University Central Hospital
Summary
The goal of this clinical trial is to investigate in healthy volunteers the mechanisms by which ethanol and lipids, the two key risk factors of steatotic liver disease (SLD), affect liver mitochondrial metabolism. The main question it aims to answer is: • Does acute administration of ethanol and lipids increase hepatic mitochondrial reductive stress as determined by orally ingested stable isotope tracer 13C-alpha-ketoisocaproate and by plasma beta-hydroxybutyrate to acetoacetate ratio (b-OHB/AcAc) in humans?
Key Details
Gender
All
Age Range
18 Years - 75 Years
Study Type
INTERVENTIONAL
Enrollment
12
Start Date
2024-09-01
Completion Date
2027-01-31
Last Updated
2024-08-19
Healthy Volunteers
Yes
Conditions
Interventions
13C-α-Ketoisocaproic Acid Breath Test
13C-α-Ketoisocaproic acid with L-Leucine soluted in citric acid drink. The solution is administered orally during metabolic visits (3.-5.).
Ethanol
6% ethanol soluted in normal saline is given intravenously during one metabolic visit.
Lipid Emulsion
Lipid emulsion, heparin and normal saline are given intravenously during one metabolic visit.
Normal Saline
Normal saline is given intravenously during one metabolic visit.
Locations (1)
Helsinki Central University Hospital
Helsinki, Uusimaa, Finland