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Antibacterial Effect of Zinc Oxide Nanoparticles on Acinetobacter Baumannii Isolated from Patients with Hospital Acquired Infections in Sohag University Hospitals, Egypt
Sponsor: Sohag University
Summary
A. baumannii is known as the most frequently isolated organism in intensive care units (ICUs), causing a variety of nosocomial infections, including pneumonia, urinary tract infections (UTIs), bacteremia as well as skin and soft tissue infections. These infections are usually associated with high mortality rates ranging between 26% among hospitalized patients and 43% among ICU patients.
Key Details
Gender
All
Age Range
Any - 90 Years
Study Type
OBSERVATIONAL
Enrollment
150
Start Date
2024-10-10
Completion Date
2025-12-30
Last Updated
2024-10-04
Healthy Volunteers
No
Interventions
Culture specific antibiotic therapy
Samples will be cultured on MacConkey agar. Morphological identifications of growth isolates by Gram staining, colony features and conventional biochemical tests. All isolates will be identified to species level using automated bacterial identification systems.
Antibiotic
Strains confirmed as A. baumannii will be examined for their different antibiotic susceptibility by modified Kirby Bauer\'s disc diffusion method on Mueller Hinton Agar.
Biofilm assessment
The biofilm formation activity of A. baumannii isolates will be tested using the microtitre plate technique
nanoparticle zinc oxide
Detection of the effect of ZnO NPs on the biofilm producer MDR A. baumannii strains using the same method
Molecular detection of antibiotic resistance
\- Evaluation the effect of ZnO NPs on the expression of some efflux pump genes, and biofilm related genes in MDR A. baumannii isolates using Real time PCR technology.
Locations (1)
Sohag university, Faculty of medicine
Sohag, Egypt