Clinical Research Directory

Inclusion Criteria: * Systemic healthy patients * Patients aged \> 25 years * Patients suffering from stage II periodontitis with grade B were included in this study, according to 2017 world workshop classification of periodontal disease. * Patients with interdental clinical attachment loss (CAL) of 3-4 mm with radiographic evidence of bone loss extending to coronal third of the root. * Radiographic bone loss (RBL) will be diagnosed using periapical parallel technique using 6 radiographic images for full mouth (3- radiographic images for maxillary arch and 3- radiographic images for mandibular arch) including anterior, canine region and posterior teeth at baseline for diagnosis. * No history of previous periodontal therapy. * No taken medications known to interfere healing or periodontal tissue health such as anticonvulsants, calcium channel blockers, or immunosuppressant drugs. * No previous periodontal surgery at involved sites. * Good oral hygiene and good compliance with the plaque control instructions following initial therapy. * Neither taken antibiotics within the last three months nor nonsteroidal anti-inflammatory drugs within the last three months * Absence of diseases or conditions that would interfere with wound healing such as diabetes mellitus or coagulation disorders Exclusion Criteria: Patients taking Vitamin D as a nutritional supplement were excluded from the study * Smoking habits * Pregnant and lactating mothers * Patients who have been received periodontal surgery in the study area during the last 6 months.