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NOT YET RECRUITING
NCT06765330
NA

Microbiome Changes After Laser and Chlorhexidine Disinfection in Caries Removal

Sponsor: Mersin University

View on ClinicalTrials.gov

Summary

The aim of this study was to investigate the effects of cavity disinfection with laser and chlorhexidine on the microbiome in cavities prepared using different caries removal methods.

Official title: Microbiome Changes Following Laser and Chlorhexidine Cavity Disinfection in Different Caries Removal Methods

Key Details

Gender

All

Age Range

18 Years - Any

Study Type

INTERVENTIONAL

Enrollment

78

Start Date

2025-05-05

Completion Date

2025-09-15

Last Updated

2025-01-09

Healthy Volunteers

Yes

Conditions

Interventions

DRUG

CHX disinfection

Each CHX cavity will be treated with disinfectant with a disposable micro brush tip and left undisturbed for 20 seconds. They will then be air-dried for 10 seconds and restore the tooth.

DEVICE

Laser Disinfection

After the caries removal procedure, the cavity will be disinfected using an Er,Cr: YSGG laser (EPIC X™, BIOLASE, USA) with 940 nm and 0.1 W output power. The laser tip will be a 400 µm tip with an energy density of 138.12 J/cm in non-contact mode (2 mm distance), 5 s/mm2 sweeping motion.

PROCEDURE

Tungsten carbide bur

Carious tissue will be completely removed from the surrounding cavity walls with a tungsten carbide bur, leaving at least 2 mm of peripheral intact tooth structure for good adaptation and sealing of the restoration.

PROCEDURE

Polymer bur

Access to the lesion will be achieved using a high-speed handpiece with a sterile high-speed bur. The polymer bur will be used with a low-speed handpiece at 5,000-10,000 RPM to remove carious dentin in sizes RA4, RA6, and RA8 according to the size of the caries lesion. The instrument will be used with a circular, light force starting from the center of the carious lesion towards the periphery as recommended by the manufacturer. Caries removal will be stopped when the instrument is macroscopically worn and blunt and can no longer remove tissue. An excavator will be used to verify if any caries remain at the base of the cavity. If necessary, a new polymer bur will be used to remove the remaining decay.

Locations (1)

Mersin University, Faculty of Dentistry, Department of Restorative Dentistry

Mersin, Yenisehir, Turkey (Türkiye)