Clinical Research Directory

Inclusion Criteria: * Patients aged 18 to 64 years. * Patients without any known systemic disease. * Patients requiring root canal treatment for prosthetic purposes (Healthy pulp group). * Patients with third molars (wisdom teeth) indicated for extraction due to orthodontic or other clinical reasons (Healthy pulp group). * Patients diagnosed with symptomatic irreversible pulpitis based on the criteria established by the American Association of Endodontists (AAE) (Symptomatic irreversible pulpitis group). * Patients scheduled for routine root canal treatment at the Department of Endodontics, Faculty of Dentistry, Kırıkkale University. * Patients who have provided written informed consent to participate in the study. Exclusion Criteria: * Patients with any known systemic disease. * Pregnant women or those suspected of being pregnant. * Patients who have used antibiotics, anti-inflammatory drugs, or antidepressants within the past 4 weeks. * Patients with significant dental plaque or calculus, gingival redness or bleeding, severe gingivitis, generalized periodontitis, or periodontal pockets deeper than 4 mm. * Patients with teeth showing internal or external root resorption. * Patients for whom complete rubber dam isolation cannot be achieved. * Patients with teeth exhibiting signs of necrosis, apical lesions, swelling, or presence of a sinus tract. * Patients with immature teeth presenting open apices. * Patients with third molars (wisdom teeth) that are not fully erupted or have been previously diagnosed with pericoronitis.