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Developing Remote Human Milk Collection as a Novel Technique for Transcriptome Sequencing
Sponsor: University of Wisconsin, Madison
Summary
This study will develop a protocol for remote human milk collection for use in gene expression research through application of ribonucleic acid (RNA) preservation reagents. The investigators hypothesize that addition of preservation reagents will preserve RNA in human milk, facilitating downstream analyses that require intact RNA. 50 lactating participants will be recruited and requested to provide reagent-mixed samples to be sent to the university through shipping services where they will be evaluated for RNA quantity, quality and profile.
Key Details
Gender
FEMALE
Age Range
18 Years - Any
Study Type
INTERVENTIONAL
Enrollment
50
Start Date
2025-11-14
Completion Date
2026-06
Last Updated
2026-02-13
Healthy Volunteers
Yes
Conditions
Interventions
Remote Human Milk Sample Collection
Two products will be evaluated for their ability to preserve RNA in human milk samples. * RNAlater (ThermoFisher©) (ratio variable) * NucleoProtect RNA (Takara©) (ratio variable)\* * Product volume mixed with human milk samples will vary by product and may be titrated as part of protocol development.
Locations (1)
University of Wisconsin
Madison, Wisconsin, United States