Clinical Research Directory

Inclusion Criteria: (For POI patients) 1. Women aged 20 to 40 years at screening visit. 2. Clinical diagnosis of premature ovarian insufficiency (based on diagnostic criteria: 25 \< FSH ≤ 40 IU/L, reduced follicle count and abnormal follicle development by ultrasound). 3. Able to provide informed consent. (For healthy volunteers) 1. Age (±2 years) and sex-matched healthy control individuals for each POI patient. 2. Able to provide informed consent. Exclusion Criteria: (For POI patients) 1. Family history of premature ovarian insufficiency. 2. Use of hormone therapy (such as HRT) within the past 6 months. 3. Serious illness (e.g., heart failure or malignancy). 4. Smoking more than 15 cigarettes per day or history of alcohol or drug addiction. 5. Inflammatory gastrointestinal diseases. 6. Chronic diseases that may affect the gut microbiota (e.g., diabetes, cirrhosis, or cardiovascular disease). 7. Blood or autoimmune diseases, or use of immunosuppressants in the past 3 months. 8. Use of antibiotics in the past 3 months before sample collection. 9. Chronic constipation. (For healthy volunteers) 1. Have a history of infertility or recurrent miscarriage. 2. Pregnant or breastfeeding. 3. Use of hormone therapy (e.g., loratadine, oral contraceptives, etc.) within the past 6 months. 4. Inflammatory gastrointestinal diseases. 5. Smoking more than 15 cigarettes per day or history of alcohol or drug addiction. 6. Chronic diseases that may affect the gut microbiota (e.g., diabetes, cirrhosis, or cardiovascular disease). 7. Blood or autoimmune diseases, or use of immunosuppressants in the past 3 months. 8. Use of antibiotics in the past 3 months before sample collection. 9. Chronic constipation. 10. Participating in other clinical trials.