Clinical Research Directory

* Inclusion criteria for patients with moderate-to-severe DED were as follows: * (1) age 18-70 years, regardless of sex; * (2) a diagnosis of DED based on the 2016 Asian Dry Eye Society guidelines and the TFOS DEWS II criteria. Moderate-to-severe DED was defined as meeting the DED diagnostic criteria and having at least one of the following: an OSDI score ≥23 (indicating symptomatic dry eye), FBUT ≤5 s, or Schirmer I test ≤5 mm/5 min; * (3) willingness and ability to comply with routine treatment and OSM transplantation during the study period. * (4) Voluntary participation in this clinical study and provision of written informed consent. * Exclusion criteria for patients with moderate-to-severe DED were as follows: * (1) history of chronic diseases; * (2) current or previous eyelid, conjunctival, or lacrimal duct disease; * (3) history of ocular surgery or contact lens wear; * (4) concurrent active infection of the eye or other systemic sites; * (5) history of recurrent viral keratitis or the presence of significant neurotrophic keratitis; * (6) use of systemic medications within the past 2 months; * (7) severe organ disease; * (8) immunodeficiency; * (9) participation in another clinical trial within the past month or current participation in another clinical trial; * (10) allergy to any component of the study device or study reagents; * (11) chronic use of medications or probiotic-containing supplements; * (12) unhealthy lifestyle habits or environmental factors (e.g., smoking or alcohol abuse); * (13) any other conditions considered incompatible with the study. * Healthy donor inclusion criteria were as follows: * (1) Age 18-50 years; * (2) The donor for OSM transplantation was a family member of the recipient and had a consanguineous (biological) relationship with the recipient; * (3) Normal structure and function of the ocular surface, no ocular diseases or related symptoms, and normal visual function. * Healthy donor exclusion criteria were as follows: * (1) infectious diseases such as HIV or hepatitis B; * (2) potential viral or other infections of the ocular surface; * (3) current or previous eyelid, conjunctival, or lacrimal duct disease; * (4) use of systemic medications, antibiotics, herbal medicines, or probiotics within the past 1 month; * (5) pregnancy or any other condition that may affect the study results.