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RECRUITING
NCT07298863
NA

The Effect of Hydration Status on Substrate Oxidation at Rest and During Light and Moderate Exercise

Sponsor: PepsiCo Global R&D

View on ClinicalTrials.gov

Summary

Metabolic flexibility is the ability to properly switch between fat and carbohydrate stores to use for energy under different conditions (rest, feeding, exercise). Impairments in metabolic flexibility, also known as metabolic inflexibility, have been suggested to be an underlying cause of metabolic disease, like type 2 diabetes. Long-term low fluid intake may increase the risk of type 2 diabetes due to insulin resistance, a form of metabolic inflexibility. Further, low fluid intake has been shown to impair the ability to switch fuel preference during exercise. While there is some evidence that low fluid intake may lead to impaired metabolic flexibility, more information is needed. Therefore, the purpose of this study is to evaluate the impact of hydration status on substrate preference at rest and during exercise at varying intensities (light and moderate). Further, we will examine whether biological sex and menstrual cycle phase impact hydration and metabolism under these same conditions.

Key Details

Gender

All

Age Range

18 Years - 35 Years

Study Type

INTERVENTIONAL

Enrollment

74

Start Date

2025-11-05

Completion Date

2026-04-30

Last Updated

2026-03-03

Healthy Volunteers

Yes

Interventions

OTHER

Exercise Trial

Perform steady-state cycling exercise for approximately 1 hour at 70-80% of maximal heart rate

OTHER

Experimental Trial

24h after exercise trial, following assigned 24h hydration plan, perform indirect calorimetry followed by 30 min of light cycling (\~50% VO2 max) then 30 min of moderate-high cycling (\~80% VO2 max).

Locations (1)

Gatorade Sports Science Institute

Valhalla, New York, United States