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Genes Associated With Bone Metabolism in the Saliva During Orthodontic Treatment
Sponsor: Kurdistan Higher Council of Medical Specialties
Summary
Understanding the biological events during fixed orthodontic treatment is essential for optimizing treatment strategies, predicting patient response, and minimizing adverse effects. Most studies on bone remodeling have used invasive sampling methods such as tissue biopsies or serum collection; these methods cannot be used for routine clinical monitoring. Saliva is a simple medium that can reflect changes in local periodontal and bone conditions, it is also non-invasive and cheap. There is little evidence about the temporal expression of genes related to bone metabolism (RANKL, OPG, ALP, TRAP, RUNX2) in saliva during orthodontic therapy. This study will help advance the understanding of biological responses during orthodontic tooth movement and explore whether saliva can be an appropriate diagnostic medium for monitoring bone remodeling in orthodontic patients
Official title: Expression of Genes Related to Bone Metabolism in Saliva of Patients During Early Fixed Orthodontic Treatment: a Prospective Clinical Study
Key Details
Gender
All
Age Range
15 Years - 25 Years
Study Type
OBSERVATIONAL
Enrollment
24
Start Date
2025-10-12
Completion Date
2026-02
Last Updated
2025-12-26
Healthy Volunteers
No
Interventions
PCR
Unstimulated whole saliva collection SOP (time of day, fasting, avoid toothbrushing immediately prior). RNA stabilization and extraction (saliva RNA kits). cDNA synthesis and quantitative RT-PCR (or RNA-seq if budget allows). Housekeeping genes for normalization (e.g., GAPDH, ACTB - validate stability in saliva). Analysis method: ΔΔCt → fold change.
Locations (1)
Hawler teaching hospital
Erbil, Ervil, Iraq