Clinical Research Directory

Inclusion Criteria: 1. Patients aged 18 to 70; 2. Direct Surgery Group (Cohort 1) : Radical surgery was performed without neoadjuvant therapy; 3. Neoadjuvant therapy group (Cohort 2) : The initial diagnosis was invasive breast cancer with confirmed axillary lymph node metastasis, and the patient was willing to undergo radical surgery at the end of treatment; 4. Plasma from patients during treatment can be obtained; 5. Be willing to sign the informed consent form. - Exclusion Criteria: 1. Be pregnant or breastfeeding; 2. Patients whose lesions have been resected; 3. Suffered from other types of malignant tumors with a clear pathological diagnosis within 5 years prior to enrollment; 4. Within the past year of enrollment, the patient had other malignant tumors suspected by imaging, but they were not confirmed by pathology; 5. Suspected distant metastatic lesions on imaging, or potential lymph node lesions that cannot be completely cured by surgery; 6. Have received any blood product infusion treatment in the past 30 days. -