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mexB Efflux Pump Gene Expression, Activity and Biofilm Formation in Clinical Isolates of Pseudomonas Aeruginosa
Sponsor: Assiut University
Summary
1. Detection of expression level of mexB efflux pump gene in multidrug resistant clinical isolates of Pseudomonas aeruginosa by real time PCR(QRT-PCR). 2. Phenotypic detection of efflux pump activity in multidrug resistant clinical isolates of Pseudomonas aeruginosa. 3. Phenotypic detection of biofilm formation and its degree (strong-moderate-weak) in multidrug resistant clinical isolates of Pseudomonas aeruginosa. 4. Relationship between expression level of mexB efflux pump gene and degree of biofilm formation in multidrug resistant clinical isolates of Pseudomonas aeruginosa.
Official title: Relationship Between mexB Efflux Pump Gene Expression, Efflux Pump Activity and Biofilm Formation in Multidrug Resistant Clinical Isolates of Pseudomonas Aeruginosa
Key Details
Gender
All
Age Range
Any - Any
Study Type
OBSERVATIONAL
Enrollment
80
Start Date
2026-04
Completion Date
2028-01
Last Updated
2026-03-17
Healthy Volunteers
No
Conditions
Interventions
Real time PCR
Clinical isolates of Pseudomonas aeruginosa will undergo RNA extraction followed by cDNA (complementary DNA) synthesis. Quantitative real-time PCR will be performed using specific primers for the mexB efflux pump gene, and SYBR Green will be used to quantify gene expression. Housekeeping genes (e.g., rpoD or 16S rRNA) will serve as internal controls. The procedure allows precise quantification of mexB expression levels in each isolate.