Clinical Research Directory

Inclusion Criteria * Healthy patients (ASA classification 1 and 2) of age range of 18-40 of both sexes would be included. * Vital mature permanent posterior teeth with deep caries approaching the pulp as seen on periapical x-ray. * Pain indicative of irreversible pulpitis (spontaneous pain or pain exacerbated by a cold stimulus which could last from a few seconds to hours), which a cold test could reproduce. * Tooth will be selected on the basis of working diagnosis i.e. successful hemostasis within 5 minutes after removal of coronal pulp. * Absence of clinical signs or symptoms suggesting a non-vital tooth such as tenderness to percussion or palpation, mobility, presence of abscess, soft tissue swelling or suppurating sinus. * Absence of radiographic changes as; furcation or periapical pathology, internal or external root resorption or calcification in the canals. * The tooth structure of selected teeth should be restorable after completion of the treatment Exclusion Criteria: * Patients with ASA classification 3, 4 ,5 and 6 would be * Patients taking antibiotics within last 2 weeks. * Teeth with pulp necrosis, cracked tooth, internal or external resorption, calcified canals, acute or chronic apical periodontitis with an associated sinus tract or periapical radiolucency. * Periodontally compromised tooth. * Uncooperative patients or failure to obtain authorization from patients.