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Bacterial Profile Changes During Different Sodium Hypochlorite Irrigation Regimens and Calcium Hydroxide Medicament in Root Canal Treatment
Sponsor: Hasanuddin University
Summary
The goal of this clinical trial is to learn how the bacterial profile in infected root canals changes during different phases of routine root canal treatment in adults with chronic apical abscess. The main questions it aims to answer are: * How does the root canal bacterial profile change before and after irrigation with 5.25 percent sodium hypochlorite or 3 percent sodium hypochlorite? * How does the bacterial profile change after calcium hydroxide medicament is placed in the root canal? Participants will: * Receive standard root canal treatment with one of two irrigation regimens (5.25 percent or 3 percent sodium hypochlorite, plus EDTA), * Have root canal samples taken three times (before any treatment, after irrigation, and after calcium hydroxide medicament), and * Have the bacteria in these samples analyzed using 16S rRNA PCR and DNA sequencing.
Official title: Bacterial Profile Changes During Root Canal Treatment With 5.25% and 3% Sodium Hypochlorite Irrigation and Calcium Hydroxide Medicament Assessed by 16S rRNA PCR
Key Details
Gender
All
Age Range
18 Years - 75 Years
Study Type
INTERVENTIONAL
Enrollment
24
Start Date
2025-10-13
Completion Date
2026-05-18
Last Updated
2026-06-05
Healthy Volunteers
No
Interventions
Root canal treatment with 5.25% sodium hypochlorite irrigation
Standard root canal procedure in which the canal is irrigated with 5.25 percent sodium hypochlorite, followed by sterile distilled water and 17 percent EDTA, then calcium hydroxide intracanal medicament and final obturation, with bacterial sampling at three time points.
Root canal treatment with 3% sodium hypochlorite irrigation
Standard root canal procedure in which the canal is irrigated with 3 percent sodium hypochlorite, followed by sterile distilled water and 17 percent EDTA, then calcium hydroxide intracanal medicament and final obturation, with bacterial sampling at three time points.
Locations (1)
Dental and Oral Hospital Hasanuddin University
Makassar, South Sulawesi, Indonesia