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Effects of Ozone Therapy on Muscle Damage
Sponsor: Inonu University
Summary
The goal of this clinical trial is to determine the effectiveness of different ozone therapy methods in enhancing recovery after muscle-damaging exercise in healthy adults, and to evaluate their impact on biochemical markers of muscle damage and fatigue. The main questions it aims to answer are: * Do ozone-based interventions accelerate recovery by reducing biochemical markers of muscle damage and fatigue following eccentric exercise? * Which ozone therapy modality provides the greatest recovery benefit? Researchers will compare three intervention groups (autohemotherapy, ozone sauna therapy, and ozonated lavender oil massage) with a control group receiving no treatment. Participants will: * Complete a standardized eccentric exercise protocol designed to induce muscle damage * Receive one of the assigned interventions or no treatment (control) * Provide blood samples at multiple time points (baseline, post-exercise, post-intervention, and 24, 48, and 72 hours follow-up) to assess recovery- related biomarkers
Official title: Effects of Ozone Therapy Modalities on Biochemical Markers of Muscle Damage Following Eccentric Exercise
Key Details
Gender
MALE
Age Range
18 Years - 30 Years
Study Type
INTERVENTIONAL
Enrollment
32
Start Date
2024-10-04
Completion Date
2025-02-20
Last Updated
2026-07-27
Healthy Volunteers
Yes
Interventions
Autohemotherapy
Autohemotherapy was performed by withdrawing 100 mL of venous blood, which was subsequently exposed to an oxygen-ozone gas mixture (5% ozone, 95% oxygen) for 5-10 minutes in an ozone-resistant, sterile container. The ozonated blood was then rein-fused intravenously over a 15-minute period in accordance with established clinical protocols
Ozone Sauna Therapy
Ozone sauna therapy was administered in a sealed cabin maintained at a tempera-ture of 45°C. Participants remained seated with the head positioned outside the cabin and the neck sealed using a polyethylene collar to prevent ozone inhalation. Each session lasted 20 minutes and was conducted at an ozone concentration of 20 µg·mL-¹
Ozonated Lavender Oil Massage
Massage with ozonated lavender oil was administered by a certified massage thera-pist and targeted the lower extremity muscle groups, including the quadriceps, ham-strings, and gluteal muscles. The intervention lasted 20 minutes in total (10 minutes per limb) and followed standardized techniques adapted from Braun and Simonson (2008).
Locations (1)
Inonu University
Malatya, Malatya, Turkey (Türkiye)