Clinical Research Directory

Inclusion Criteria: * Adult men or women (18-50 years) * Diagnosis of MASLD (grade 1 or 2 of steatosis confirmed by ultrasound) * Body mass index (BMI) = 25-34.9 kg/m² * Providing written informed consent Exclusion Criteria: * Pregnancy, lactation, or plans to get pregnant during the next three months. * Liver disease (viral hepatitis, autoimmune liver disease, cirrhosis, drug-induced hepatotoxicity, or alcoholic fatty liver disease), heart or renal failure, kidney stones, any neoplasia, inflammatory disease, hypothyroidism, hypercortisolism, or hypertension * Taking drugs affecting glucose or lipid metabolism, folate supplements, anti-obesity medications, weight-loss diets, or dietary supplements * Lifestyle factors known to impact folate status (current smoking, alcohol intake, recreational drug use) * Pre-existing conditions affecting folate status (malabsorptive or inflammatory bowel diseases, active celiac disease, gastric bypass surgery, atrophic gastritis, epilepsy, advanced liver disease, kidney dialysis, type 1 or 2 diabetes mellitus, or sickle cell trait/anemia) * Medications that interfere with B-vitamin metabolism (chloramphenicol, methotrexate, metformin, sulfasalazine, phenobarbital, phenytoin, primidone, triamterene, barbiturates)