Clinical Research Directory

Retrospective Cohort Inclusion Criteria: * Age ≥ 18 years; * Histologically confirmed diagnosis of colorectal adenocarcinoma; * Radiological evidence of metastatic or locally advanced unresectable disease; * First-line treatment for metastatic disease already completed or ongoing according to standard clinical practice; * Availability of stored biological samples collected at least at one of the protocol-defined time points (baseline, post-treatment, disease progression); * Informed consent acquisition will be performed in accordance with Article 110-bis of the Italian Privacy Code. Retrospective Cohort Exclusion Criteria: * Absence of suitable biological samples or samples unsuitable for molecular analyses; * Incomplete clinical data preventing the performance of the planned analyses; * Insufficient quality of stored biological samples for the assessment of EV-RNA and EV-PROT analyses. Prospective Cohort Inclusion Criteria: * Age ≥ 18 years; * Histologically confirmed diagnosis of colorectal adenocarcinoma; * Radiological evidence of unresectable metastatic disease; * Patients not previously treated for metastatic disease (prior completed adjuvant/neoadjuvant therapy is allowed if completed ≥ 6 months before study enrollment); * Indication for first-line treatment with chemotherapy ± biological therapy according to standard clinical practice; * Ability to understand the study procedures and provide written informed consent. Prospective Cohort Exclusion Criteria: * Previous systemic treatment for metastatic disease (except prior adjuvant/neoadjuvant therapy as specified above); * Ongoing pregnancy or breastfeeding; * Legal incapacity or limitation in the ability to provide informed consent.