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Hippo Signaling Pathway Proteins in Gingival Crevicular Fluid of Individuals With Periodontal Disease
Sponsor: Inonu University
Summary
Periodontal disease is a chronic inflammatory condition of the tooth-supporting tissues in which the host inflammatory response drives the destruction of alveolar bone and periodontal attachment. The Hippo signaling pathway is a conserved regulatory network that controls cell proliferation, apoptosis, and bone homeostasis, but its role in human periodontal disease remains unknown. This observational, case-control study compared the levels of Hippo signaling pathway proteins (YAP, TAZ, MST1, TEAD, LATS1, MAP4K4), the anti-apoptotic protein BCL-2, and the bone metabolism marker alkaline phosphatase (ALP) in gingival crevicular fluid across 75 systemically healthy participants grouped as periodontally healthy, gingivitis, or stage 3 grade B periodontitis. The aim was to determine whether these proteins are differentially expressed across periodontal health and disease states and whether they correlate with markers of apoptosis and bone metabolism.
Official title: Hippo Signaling Pathway Proteins and Their Correlation With Markers of Apoptosis and Bone Metabolism in the Gingival Crevicular Fluid of Individuals With Periodontal Disease
Key Details
Gender
All
Age Range
18 Years - 65 Years
Study Type
OBSERVATIONAL
Enrollment
75
Start Date
2024-01-01
Completion Date
2025-04-01
Last Updated
2026-08-12
Healthy Volunteers
Yes
Conditions
Interventions
ELISA Biomarker Analysis
Gingival crevicular fluid samples were collected from each participant using standardized paper strips placed into the periodontal pocket for a fixed interval, and fluid volume was measured electronically. Samples were pooled per participant and stored until analysis. The levels of Hippo signaling pathway proteins (YAP, TAZ, MST1, TEAD, LATS1, MAP4K4), the anti-apoptotic protein BCL-2, and the bone metabolism marker alkaline phosphatase were quantified by enzyme-linked immunosorbent assay according to the manufacturers' instructions. Standard curves were generated individually for each biomarker, and results were expressed both as total amounts and as concentrations to account for differences in fluid volume between groups.
Locations (1)
Inonu University Faculty of Dentistry
Malatya, Turkey (Türkiye)