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High-Intensity Interval Training and Monocyte Epigenetic Adaptation After Detraining
Sponsor: Saif Rasheed Ghanim
Summary
This randomized, work-matched training-detraining-rechallenge study evaluated whether repeated high-intensity interval training produces persistent functional and epigenetic adaptations in human monocytes. Eighty healthy physically inactive adults aged 18-30 years were randomly assigned in a 2:2:1 ratio to high-intensity interval training (HIIT), moderate-intensity continuous training (MCT), or a non-training control group. HIIT and MCT completed 6 weeks of supervised cycling training matched for external mechanical work, followed by 4 weeks without prescribed structured training. All groups subsequently completed a standardized 30-minute cycling rechallenge. The primary outcome was the between-group difference in change in ex-vivo LPS-stimulated TNF-alpha release from baseline to the post-detraining assessment. Secondary outcomes included IL-6 release, H3K18 lactylation, monocyte subset distribution, blood lactate exposure, chromatin accessibility, H3K18la CUT\&Tag occupancy, and RNA-seq transcriptional profiling.
Official title: Repeated High-Intensity Exercise Induces Persistent H3K18 Lactylation and Trained Immunity in Human Monocytes: A Randomized Work-Matched Training-Detraining-Rechallenge Trial
Key Details
Gender
All
Age Range
18 Years - 30 Years
Study Type
INTERVENTIONAL
Enrollment
80
Start Date
2026-01-14
Completion Date
2026-07-02
Last Updated
2026-08-18
Healthy Volunteers
Yes
Conditions
Interventions
High-Intensity Interval Training
Supervised cycling HIIT performed three sessions per week for 6 weeks. Each session included a 5-minute warm-up at 30% MAP, four 4-minute intervals at 85% MAP separated by three 3-minute active recovery periods at 40% MAP, and a 5-minute cool-down at 30% MAP.
Moderate-Intensity Continuous Training
Supervised moderate-intensity continuous cycling performed three sessions per week for 6 weeks. Each session included a 5-minute warm-up at 30% MAP, 31 minutes and 16 seconds of continuous cycling at 55% MAP, and a 5-minute cool-down at 30% MAP. The protocol was designed to match the external mechanical work performed by the HIIT group.
Locations (1)
Exercise Physiology Laboratory, College of Physical Education and Sports Sciences, University of Samarra
Samarra, Muhafazat Salah ad Din, Iraq