Clinical Research Directory
Browse clinical research sites, groups, and studies.
Gene Expression Analysis of Biofilm Formation and Antimicrobial Resistance in Clinical Methicillin-Resistant Staphylococcus Aureus
Sponsor: Sohag University
Summary
Staphylococcus aureus are Gram-positive cocci that asymptomatically colonize the skin and mucosal surfaces of approximately 20-30% of healthy individuals . Although they commonly exist as a harmless commensal organisms, S. aureus are one of the most clinically significant opportunistic pathogens, causing a wide range of community and healthcare-associated infections. These infections vary from superficial skin and soft tissue infections to severe invasive diseases, including bacteremia, infective endocarditis, pneumonia, osteomyelitis, and device-associated infections .
Official title: Gene Expression Analysis of Biofilm Formation and Antimicrobial Resistance in Clinical Methicillin-Resistant Staphylococcus Aureus Isolates From Surgical Site Infections Exposed to Probiotic Cell-Free Supernatant
Key Details
Gender
All
Age Range
6 Months - 90 Years
Study Type
OBSERVATIONAL
Enrollment
100
Start Date
2026-09-20
Completion Date
2027-11-02
Last Updated
2026-09-01
Healthy Volunteers
No
Interventions
swab from lesion
* Direct smear with Gram stain to identify the morphology of Staphylococcus aureus. * culture of the samples on different culture media such as nutrient agar, blood agar, and Mannitol salt agar.
Biochemical reactions
biochemical reactions: catalase test will be done to confirm diagnosis of Staphylococcus aureus.
Antibiotic sensitivity
Antibiotic sensitivity test will be done using different antibiotics according to CLSI 2026.
Molecular detection of antibiotic resistance
Assessment of the expression of selected biofilm-related genes (icaA and fnbA) and antibiotic resistance-related genes (mecA and femA) using quantitative real-time PCR (qRT-PCR) before and after treatment with probiotic cell-free supernatant (CFS).