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Effects of Acute Exercise in Fasting and Fed States in Individuals With Different Aerobic Fitness Levels
Sponsor: Hacettepe University
Summary
The aim of this study is to investigate the effects of aerobic fitness level, feeding status, and acute exercise on whole-body metabolic fuel utilization and mitochondrial function in peripheral blood mononuclear cells (PBMCs) in healthy young men. The main questions it aims to answer are: 1. Do individuals with high and low aerobic fitness levels differ in fat and carbohydrate oxidation at rest and during exercise following an overnight fast, and do their PBMC mitochondrial responses to acute exercise differ? 2. Does feeding alter fat and carbohydrate oxidation at rest and during exercise compared with the fasted state in individuals with high aerobic fitness? 3. Are PBMC mitochondrial respiration parameters and their exercise-induced changes associated with fat and carbohydrate oxidation during fasted exercise?
Official title: The Effect of Acute Exercise in the Fasted and Fed States on Peripheral Blood Mononuclear Cells and Metabolic Fuel Utilization in Individuals With Different Aerobic Fitness Levels
Key Details
Gender
MALE
Age Range
18 Years - 25 Years
Study Type
INTERVENTIONAL
Enrollment
30
Start Date
2026-11-15
Completion Date
2027-12-15
Last Updated
2026-09-10
Healthy Volunteers
Yes
Interventions
Acute Moderate-Intensity Cycling Exercise
Participants will perform 30 minutes of continuous cycling exercise on a cycle ergometer at an intensity corresponding to 65% of their individual maximal oxygen uptake (VO2max). Both aerobic fitness groups will complete the exercise following an overnight fast. Participants in the high aerobic fitness group will also repeat the exercise one hour after consuming an ad libitum meal. Respiratory gas exchange will be measured throughout exercise to calculate fat and carbohydrate oxidation, and rating of perceived exertion will be recorded every five minutes using the Borg 6-20 scale.
Ad Libitum Mixed Meal
One hour after completing the fasted exercise test, participants in the high aerobic fitness group will be provided with an ad libitum mixed meal consisting of pasta and meatballs and will be allowed to eat until comfortably full. One hour after the meal, resting metabolic rate measurement and the 30-minute cycling exercise test at 65% of VO2max will be repeated under the fed condition. No blood samples will be collected during the fed condition.
Locations (1)
Hacettepe University, Faculty of Sport Sciences
Ankara, Ankara, Turkey (Türkiye)