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NCT07852598

A Metagenomic Next-generation Sequencing (mNGS)-Guided Sequential Culture Strategy for Postoperative Cruciate Ligament Reconstruction Infection (CLRI): a Retrospective Cohort Study

Sponsor: First Affiliated Hospital of Fujian Medical University

View on ClinicalTrials.gov

Summary

This retrospective cohort study evaluates a sequential diagnostic strategy for patients with postoperative cruciate ligament reconstruction infection (CLRI). Diagnosing these infections is often difficult because conventional laboratory cultures can easily miss fastidious or slow-growing microorganisms. This study investigates whether using a rapid DNA-based test called metagenomic next-generation sequencing (mNGS) to guide and customize subsequent laboratory culture conditions can improve the detection rate of viable, live pathogens. The study includes 50 patients with CLRI and 30 patients with aseptic failure (non-infectious complications). The ultimate goal is to determine if this mNGS-guided modified culture strategy can help doctors identify the exact infection-causing germs more accurately, thereby allowing them to provide targeted, individualized antibiotic and surgical treatments.

Key Details

Gender

All

Age Range

Any - Any

Study Type

OBSERVATIONAL

Enrollment

80

Start Date

2015-01-01

Completion Date

2026-02-05

Last Updated

2026-10-01

Healthy Volunteers

No

Conditions

Cruciate Ligament Reconstruction InfectionSeptic ArthritisSurgical Wound InfectionJoint Infection

Interventions

DIAGNOSTIC_TEST

Metagenomic next-generation sequencing (mNGS)

Pre-therapy synovial fluid was analyzed by mNGS to rapidly detect microbial nucleic acids and identify potential pathogens.

DIAGNOSTIC_TEST

mNGS-guided modified culture

A subsequent synovial fluid culture protocol customized based on pre-therapy mNGS results, utilizing specialized media, prolonged incubation, or specific atmospheric conditions to improve viable pathogen recovery.

DIAGNOSTIC_TEST

Conventional microbiological culture

Routine aerobic and anaerobic microbiological culture methods used as the standard baseline test.

Locations (1)

The First Affiliated Hospital of Fujian Medical University

Fuzhou, Fujian, China