The Metabolic Effects of Oral Tributyrin Administration
The goal of this double-blinded randomized clinical cross-over trial is to investigate the metabolic and appetite regulating effects of oral tributyrin (a butyrate pro-drug) administration vs placebo.
The main questions it aims to answer are:
* Does tributyrin have glucose lowering properties in humans?
* Does tributyrin reduce appetite in humans?
A sample size calculation estimated a minimum of 10 participants. We expect to include 12 participants in this study. Tributyrin or placebo is ingested once or twice daily for 2x2 weeks, separated by a washout period of minimum 2 weeks. On trial day following each 2-week period we assess:
* Postprandial blood sugar levels
* Hunger sensations (questionnaire)
* Enteroendocrine hormone secretion (GLP-1, GIP, PYY, LEAP2, ghrelin)
* Cardiac function (echocardiography) - An exploratory study evaluating the hemodynamic effects assessed by echocardiography is anticipated to be published separately.
* Caloric intake (ad libitum meal test)
* Gastric emptying rate (acetaminophen test)
* Resting metabolic rate (indirect calorimetry).
While at home, subjects will wear a sleep monitoring device for three consecutive nights and deliver fecal samples for analysis of short-chain fatty acid content and microbiota composition.
Gender: All
Ages: 18 Years - Any
